3/15/2023 0 Comments Incucyte software for mac -windowsIn vitro Cell Viability by CellProfiler® Software as Equivalent to MTT Assay. Conclusion As a stable and reliable method,FDA is feasible for cell variability detection under varied conditions. As to H 2 O 2 injury assay,the sensitivity of FDA method was superior to MTT on the higher concentration H 2 O 2 treatment due to a relative shorter duration for detection. On cell viability detection,the result of FDA method was equivalent to MTT method. The optimum concentration of final FDA in this study was 10-30 μg/ml. Results Within 0-30 minutes,the fluorescence- cell number coefficient of FDA assay increased with duration and reached 0.99 in 27-30 minutes. We also compared the results of FDA with methyl thiazolyl(MTT) in terms of cell numbers and H 2 O 2 injury. The effects of FDA incubation duration,concentration,and other factors on the assay's accuracy and stability were assessed. After incubated with FDA,the plate was detected by fluorescence microplate analyzer. Methods Cells were seeded in a 96-well culture plate until detection. Objective To investigate the application of the fluorescein diacetate (FDA) microplate assay in cell viability detection. Fluorescein Diacetate Microplate Assay in Cell Viability Detection.Ĭhen, Xi Yang, Xiu-Ying Fang, Lian-Hua DU, Guan-Hua
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